Citrate Mannitol Agar Medium for Separating Escherichia coli Paracolons from Shigella
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چکیده
منابع مشابه
Citrate mannitol agar medium for separating Escherichia coli paracolons from Shigella.
A culture medium is described which not only differentiates Shigella from Escherichia coli but also serves as the basis for an extremely simple test which specifically identifies E. coli. A test that will identify an organism as E. coli is more useful than one that merely groups this species with other non-Shigella organisms. The theoretical basis for specificity of the test for E. coli and oth...
متن کاملComparison of acetate differential agar with sellers citrate-mannitol-agar.
A total of 245 isolates of Escherichia coli (paracolons) were employed in comparing the use of acetate differential agar (ADA) with the simple test described by Sellers to eliminate these organisms from consideration as enteric pathogens. At 18 hr after the media were inoculated, 83% of the isolates produced a positive citrate reaction on Sellers citrate-mannitol-agar (SCM) and 7% gave a positi...
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D-Mannitol is the most widely distributed of the naturally occurring hexahydric alcohols, and the compound has been known since the early part of the nineteenth century. It is found as a reserve material in some algae and many higher plan&. In some lower fungi it is the product of the fermentation of sugars. Finally, mannitol is utilized for growth and fermented by a large number of species of ...
متن کاملThe mannitol repressor (MtlR) of Escherichia coli.
The mannitol operon of Escherichia coli, encoding the mannitol-specific enzyme II of the phosphotransferase system (Mt1A) and mannitol phosphate dehydrogenase (Mt1D), is here shown to contain a single additional downstream open reading frame which encodes the mannitol repressor (Mt1R). Mt1R contains 195 amino acids and has a calculated molecular weight of 21,990 and a calculated pI of 4.5. It i...
متن کاملCitritase, the Citrate-splitting Enzyme from Escherichia Coli
Two distinct and independent pathways for the breakdown of citric acid are now generally recognized to operate in bacteria. These include (a) the stepwise degradation of citrate via the Krebs cycle reactions (l-3) and (b) the breakdown of citrate into a Cz and Ck unit not involving CoAl (4-6). A third conceivable pathway of citrate breakdown would be via a reversal of the condensing enzyme (req...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1967
ISSN: 0003-6919
DOI: 10.1128/am.15.4.731-732.1967